Effect of propofol upon brain after whole-body hyperthermia in rats

• Published in: Zhong hua yi xue za zhi Vol. 89; no. 33; p. 2356
• Main Authors: Zhao, Xin, Jin, Yan-Wu, Wang, Duan-Yu, Wang, Zhi-Gang
• Format: Journal Article
• Language: Chinese
• Published: China 08.09.2009
• Subjects: Animals; Apoptosis - drug effects; Brain - drug effects; Brain - pathology; Disease Models, Animal; Heat Stress Disorders; Hippocampus - cytology; Male; Neurons - ultrastructure; Propofol - pharmacology; Rats; Rats, Wistar
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2009.33.014

To investigate the effect of propofol upon acute cerebral insult during whole-body hyperthermia (WBH) in rats. Sixty male Wistar rats were randomly divided into 6 groups. All the animals were trained with place navigation test of Morris water maze (MWM) for 5 days pre-study. After training, some rats received anesthesia only but not WBH treatment as control, other rats were anesthetized with intraperitoneal injection of 3.0 ml/kg of chloral hydrate and 100 or 150 mg/kg of propofol followed with WBH process (keeping rat core temperature at 42 degrees C for 30 min). Space memory was checked using spatial probe test 24 h after WBH intervention. Rat brain was then removed in 24 h post-WBH to separate hippocampal regions for the detection of neuronal apoptosis with TUNEL method and ultramicrostructural changes by electron microscope. The escape latency were smaller in groups B0, C0, D0, C and D than that in group B (P < 0.05) while smaller in groups B0, C0, D0 than in groups B, C, D respectively. The amount of apo