Coreceptor usage of human immunodeficiency virus-1 in primary infection through sexual contact transmission

• Published in: Zhong hua yi xue za zhi Vol. 91; no. 21; p. 1457
• Main Authors: Wang, Yan-hai, Han, Xiao-xu, An, Ming-hui, Dai, Di, Zhao, Bin, Shen, Shu-xu, Chu, Zhen-xing, Xu, Jun-jie, Jiang, Yong-jun, Shang, Hong
• Format: Journal Article
• Language: Chinese
• Published: China 07.06.2011
• Subjects: Acquired Immunodeficiency Syndrome - transmission; Acquired Immunodeficiency Syndrome - virology; Adult; env Gene Products, Human Immunodeficiency Virus - genetics; HIV-1 - genetics; HIV-1 - metabolism; Humans; Male; Middle Aged; Prospective Studies; Receptors, CCR5 - metabolism; Receptors, CXCR4 - metabolism; Sexually Transmitted Diseases, Viral - virology; Young Adult
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2011.21.005

To evaluate the coreceptor usage characters and evolution of human immunodeficiency virus-1 (HIV-1) primary infection. A total of 12 HIV-1 primary infection cases were recruited from a high-risk population for this prospective cohort trial. The blood samples were collected at the earliest time after infection and after a viral setpoint respectively. RNA was extracted from plasma. The env genes were amplified through reverse transcription-PCR (RT-PCR) and nested PCR. And the env fragments were ligated to T vector and then sequenced. The pattern of coreceptor usage was predicted with five different online tools. Among them, 11 were predicted as CCR5 virus transmission and 1 as CXCR4 virus transmission. The viruses in 1 case were detected as a switch of coreceptor usage from CCR5 to CXCR4. The consistency of five different prediction rules was 88.3%. Although the CCR5 usage virus is predominant in primary infection through sexual transmission, CXCR4 usage virus can also be detected. A switch of coreceptor usage