Serum proteomics profiling for diagnosis of myasthenia gravis

• Published in: Zhong hua yi xue za zhi Vol. 89; no. 31; p. 2184
• Main Authors: Wu, Guo-Yong, Pang, Jing-Zhuo, Cheng, Chao, Lu, Jian-Jun, Ma, Jun, Gu, Yong, Zhong, Fu-Tian, Luo, Hong-He
• Format: Journal Article
• Language: Chinese
• Published: China 18.08.2009
• Subjects: Adolescent; Adult; Blood Proteins - analysis; Case-Control Studies; Child; Child, Preschool; Female; Humans; Male; Middle Aged; Myasthenia Gravis - blood; Myasthenia Gravis - diagnosis; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Young Adult
• DOI: 10.3760/cma.j.issn.0376-2491.2009.31.007
• ISSN: 0376-2491

To identify the significant protein peaks and establish the diagnostic model of myasthenia gravis (MG) by serum proteomics profiling analysis. The serum samples from 56 MG patients and 16 healthy controls were detected by the technology of surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). The differentially expressed protein peaks were identified to establish a MG diagnostic model. And preliminary validation was performed. Thirty-eight specific protein peaks with significant differences were found in the serum protein pattern of 56 MG patients and 16 healthy controls. Systemic optimization identified 2 protein peaks of M4168.94 and M1122.57. And they were used to build the MG diagnostic model of differentiating 56 cases from 16 controls. The serum protein profiling can be a novel, effective and sensitive tool to screen for MG-related protein peaks and establish a diagnostic model.