Serum proteomics profiling for diagnosis of myasthenia gravis

To identify the significant protein peaks and establish the diagnostic model of myasthenia gravis (MG) by serum proteomics profiling analysis. The serum samples from 56 MG patients and 16 healthy controls were detected by the technology of surface-enhanced laser desorption ionization time-of-flight...

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Published inZhong hua yi xue za zhi Vol. 89; no. 31; p. 2184
Main Authors Wu, Guo-Yong, Pang, Jing-Zhuo, Cheng, Chao, Lu, Jian-Jun, Ma, Jun, Gu, Yong, Zhong, Fu-Tian, Luo, Hong-He
Format Journal Article
LanguageChinese
Published China 18.08.2009
Subjects
Adolescent
Adult
Blood Proteins - analysis
Case-Control Studies
Child
Child, Preschool
Female
Humans
Male
Middle Aged
Myasthenia Gravis - blood
Myasthenia Gravis - diagnosis
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Young Adult
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Summary:To identify the significant protein peaks and establish the diagnostic model of myasthenia gravis (MG) by serum proteomics profiling analysis. The serum samples from 56 MG patients and 16 healthy controls were detected by the technology of surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). The differentially expressed protein peaks were identified to establish a MG diagnostic model. And preliminary validation was performed. Thirty-eight specific protein peaks with significant differences were found in the serum protein pattern of 56 MG patients and 16 healthy controls. Systemic optimization identified 2 protein peaks of M4168.94 and M1122.57. And they were used to build the MG diagnostic model of differentiating 56 cases from 16 controls. The serum protein profiling can be a novel, effective and sensitive tool to screen for MG-related protein peaks and establish a diagnostic model.
ISSN:0376-2491
DOI:10.3760/cma.j.issn.0376-2491.2009.31.007