Elimination of leukemic CD34+ progenitor cells by using cytotoxic T lymphocytes specifically targeting WT1-derived peptide: an experimental study in vitro

• Published in: Zhong hua yi xue za zhi Vol. 88; no. 48; p. 3401
• Main Authors: Gu, Wei-ying, Chen, Zi-xing, Qiu, Guo-qiang, Hu, Shao-yan, Jia, Li, Wu, Wei, He, Jun, Cen, Jian-nong, He, Bai
• Format: Journal Article
• Language: Chinese
• Published: China 30.12.2008
• Subjects: Adult; Antigens, CD34; Bone Marrow Cells - immunology; Case-Control Studies; Cell Line, Tumor; Female; Flow Cytometry; Genes, Wilms Tumor; Granulocyte-Macrophage Progenitor Cells - immunology; HLA-A Antigens - immunology; HLA-A2 Antigen; Humans; Leukemia - therapy; Male; Middle Aged; Peptides - genetics; T-Lymphocytes, Cytotoxic - immunology; WT1 Proteins - genetics
• ISSN: 0376-2491

To investigate the effects of targeting elimination of the leukemic CD34+ progenitor cells by using cytotoxic T lymphocytes (CTLs) specifically against Wilms tumor gene (WT1)-derived peptide. A 9-mer WT1 peptide (CMTWNQMNL) containing HLA-A * 0201-binding anchor motifs was synthesized. The suspended cells were collected from the culture of the peripheral blood mononuclear cells and divided into 2 groups: Group D (pure T cells) and Group C (IL2 + T cells). The dendritic cells (DCs) generated from the peripheral blood mononuclear cells of an HLA-A* 0201-positive healthy donor were repeatedly loaded with WT1 peptide so as to elicit cytotoxic T cells (CTLs) specifically for WT1 peptide, and restricted by HLA-A * 0201 (Group A). The specific lysis effects of WT1 peptide specific CTLs upon leukemic bone marrow CD34+ progenitor cells positively expressing WT1 (3 being HLA-A * 0201(+) and 3 being HLA-A*0201(-)), peripheral blood CD34+ cells from healthy persons (2 being HLA-A * 0201(+) and 1 being HLA-A * 0201(-)), a