Nanoparticle-mediated endostatin gene therapy targeting hepatocellular carcinoma utilizing heat-inducible promoter

• Published in: Zhong hua yi xue za zhi Vol. 89; no. 12; p. 795
• Main Authors: Zhou, Jia-Jia, Chen, Ru-Fu, Li, Zhi-Hua, Zhou, Quan-Bo, Tang, Qi-Bin, He, Xiao-Yu, Lu, Hong-Wei, Guo, Ning
• Format: Journal Article
• Language: Chinese
• Published: China 31.03.2009
• Subjects: Animals; Carcinoma, Hepatocellular - therapy; Cell Line, Tumor; Endostatins - genetics; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Hot Temperature; Humans; Liver Neoplasms, Experimental - therapy; Mice; Mice, Inbred BALB C; Mice, Nude; Nanoparticles - therapeutic use; Promoter Regions, Genetic; Transfection
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2009.12.002

To investigate the inhibitory effect of nanoparticle-mediated endostatin gene therapy on hepatocellular carcinoma xenografts combined with local hyperthermia utilizing heat-inducible promoter. Heat-inducible HSP70B promoter and fusion gene of Endo/EGFP were cloned into pcDNA3.1 (+) plasmid, thus obtaining recombinant plasmid of pcDNA3.1 (+)/HSP70-Endo/EGFP using restriction endonucleases BglII/HindIII and EcoRI/SalI. The nanoparticles polylactide-grafted dextran copolymer (DEX-g-PLA) encapsulating the recombinant plasmid DNA were prepared by the method of emulsification and evaporation of organic solvent, and the surface shape of nanoparticles was observed by transmission electron microscope. Human hepatocellular cells of the lines HepG2 and ECV304 were cultured and transfected with the recombinant plasmid utilizing the nanoparticles. Following thermal induction at 37 degrees C, 39 degrees C, 41 degrees C, 43 degrees C, and 45 degrees C for 30 min, the expression of enhanced green fluorescent protein (EGFP) w