Spatial characteristics of microbial groups associated with the groundwater flow in a small watershed

Few reports of microbial groups associated with the groundwater flow system are available in China. 16S rRNA gene library was constructed by the cultured-independent approach to investigate gene sequences of microorganism in groundwater samples from the recharge (R), intermediate (M) and discharge (...

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Bibliographic Details
Published inHuanjing kexue Vol. 31; no. 10; p. 2299
Main Authors Huang, Xiao-Lan, Chen, Jian-Yao, Xie, Li-Chun, Fu, Cong-Sheng, Zhou, Shi-Ning
Format Journal Article
LanguageChinese
Published China 01.10.2010
Subjects
Bacteria - classification
Bacteria - genetics
Biodiversity
China
Fresh Water - microbiology
Population Dynamics
RNA, Ribosomal, 16S - genetics
Water Microbiology
China
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Summary:Few reports of microbial groups associated with the groundwater flow system are available in China. 16S rRNA gene library was constructed by the cultured-independent approach to investigate gene sequences of microorganism in groundwater samples from the recharge (R), intermediate (M) and discharge (D) zones of an experimental watershed at Zhuhai campus of Sun Yat-sen University. Proteobacterium, Candidate division OPx, uncultured archaeon (uncultured Crenarchaeote and Euryarchaeote) and Actinobacterium are found predominant in all these three zones with the Proteobacterium accounting for 23.21%, 36.21%, and 28.84% in R, M, D zone respectively. The other predominant microbial groups were identified for varied zones, e. g. Eubacterium and Nitrospira in the R wells, Eubacterium and Acidobacterium in the M wells, and Bacteroidetes bacterium in the D wells. Linkages and potential evolution of microbial groups among three zones were examined by using the genetic neighbor-joining tree. Environmental adaptation along
ISSN:0250-3301