Partial characterization of three beta -defensin gene transcripts in river buffalo and cattle

• Published in: African journal of biotechnology Vol. 10; no. 55; pp. 11388 - 11396
• Main Authors: Mossallam, AAA, El Nahas, SM, Sabry, N M, Mahfouz, E R, Hassan, A A
• Format: Journal Article
• Language: English
• Published: 21.09.2011
• Subjects: Bos taurus; Bubalus bubalis; Africa
• ISSN: 1684-5315; 1684-5315

In this study, the tracheal tissues from Egyptian river buffalo and cattle were screened for the presence of three bovine beta -defensin gene transcripts. Three primer pairs were designed on the basis of published Bos taurus sequences for partial amplification of beta -defensin 4, beta -defensin 10 and beta -defensin 11 complementary DNA (cDNA). The amplified cDNA products of the three genes in both buffalo and cattle were sequenced. The sequences were analyzed to verify gene identity and to identify differences with the corresponding buffalo (Bubalus bubalis bubalis) and/or cattle (Bos taurus) beta -defensin mRNAs published sequences in the GenBank database. beta -Defensin 4 and beta -defensin 10 primer pairs amplified cDNA sequences in buffalo and cattle that corresponded to those mRNAs of the two genes in GenBank database with nucleotide percentage homology of 83 and 84% for beta -defensin 4, and 87 and 90% for beta -defensin10, respectively. The translated protein sequences obtained for buffalo and cattle showed protein percentage similarity of 86 and 81% for beta -defensin 4, and 87.5 and 87% for beta -defensin 10 with the corresponding proteins of B. bubalis bubalis and/or B. taurus in GenBank database. On the other hand, cDNA sequences amplified by beta -defensin 11 primer pair in both buffalo and cattle corresponded more to lingual antimicrobial peptide (LAP) mRNAs of B. bubalis bubalis and B. taurus (94 and 82% nucleotide similarity and 92 and 77% translated-protein similarity) rather than beta -defensin 11 mRNA of B. taurus (68 and 66% nucleotide similarity and 74 and 65.5% translated-protein similarity).