Quantitative Analysis of Metabolic Profile and Tamm-Horsfall Protein in Pediatric Stone Patients

• Published in: Renal Stone Disease 2 (AIP Conference Proceedings Volume 1049) Vol. 1049; pp. 324 - 327
• Main Authors: Kogan, M I, Matishov, D G, Shin, E F, Sizonov, V V
• Format: Journal Article
• Language: English
• Published: 01.01.2008
• ISBN: 0735405778; 9780735405776
• ISSN: 0094-243X
• DOI: 10.1063/1.2998047

According to some studies, the secretion of urinary glycoprotein Tamm-Horsfall protein (uromodulin) plays a significant part in the suppression of calcium nephrolith formation. The aim of the present study was to detect correlation of Tamm-Horsfall protein with soluble Ca2+ in the urine of urolithic patients and compare it with a control group, in order to estimate the degree of the disease progress, as well as the prognosis for the disease. The research included 29 urolithic patients, aged ,0+/-0, 2 y. The content of soluble Ca2+ in fresh urine was estimated by the method of capillary zone electrophoresis. The level of Tamm-Horsfall protein in urine was measured from cryoprecipitate using SDS polyacrylamide electrophoresis and semiquantitative analysis. Urine from the urolithic patients was consistently higher in Ca2+ and in Tamm-Horsfall protein concentrations than urine from controls, at all periods of the day.