Production of beta -Glucosidase by Penicillium purpurogenum

The fungus Penicillium purpurogenum was found to produce intracellular beta -glucosidase. Maximum activity of beta -glucosidase was observed on sucrose. Various cultural parameters of cultivation of P. purpurogenum for production of beta -glucosidase were optimized. Maximum enzyme content was observ...

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Bibliographic Details
Published inBrazilian journal of microbiology Vol. 36; no. 2; pp. 170 - 176
Main Authors Dhake, AB, Patil, M B
Format Journal Article
LanguageEnglish
Published 01.04.2005
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Summary:The fungus Penicillium purpurogenum was found to produce intracellular beta -glucosidase. Maximum activity of beta -glucosidase was observed on sucrose. Various cultural parameters of cultivation of P. purpurogenum for production of beta -glucosidase were optimized. Maximum enzyme content was observed after 96 hours of cultivation at 30 degree ; C. Addition of amino acids histidine and cysteine induced beta -glucosidase synthesis to certain extent. The optimum temperature and pH for beta -glucosidase activity was 50 degree ; C and 5.5 respectively. beta -glucosidase of P.purpurogenum shows stability at pH 2 thus it could be an ideal enzyme for debittering in fruit juice and wine industries.
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ISSN:1517-8382