Expression and characterization of FNRD in cyanobacterium Synechococcus sp. PCC 7002

The petHL genes under the control of Lac and Kan promoters were transformed into Synechococcus sp. PCC 7002, respectively. Both of the petHL genes are integrated into the cyanobacterium chromosomes, which is inferred from the results of Southern blot analysis. Western blot analysis results show that...

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Bibliographic Details
Published inWei sheng wu hsüeh pao Vol. 45; no. 5; p. 716
Main Authors Li, Rong-gui, Yu, Xin, Zhao, Jin-dong
Format Journal Article
LanguageChinese
Published China 2005
Subjects
Blotting, Southern
Blotting, Western
Ferredoxin-NADP Reductase - analysis
Ferredoxin-NADP Reductase - chemistry
Ferredoxin-NADP Reductase - physiology
Photosynthesis
Synechococcus - enzymology
Synechococcus - genetics
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Summary:The petHL genes under the control of Lac and Kan promoters were transformed into Synechococcus sp. PCC 7002, respectively. Both of the petHL genes are integrated into the cyanobacterium chromosomes, which is inferred from the results of Southern blot analysis. Western blot analysis results show that both petHL genes are expressed in the transformed cells, and Kan promoter is more effective than Lac promoter. The FNRD in vivo shows the same stability as that of FNR holoenzyme. Some FNRD molecules are probably acylated as judged by the result of Triton X-114 phase partition test. FNRD in vivo might act as a component in photosynthetic electron transport chain, which increases the photosynthetic oxygen evolution rate.
ISSN:0001-6209