Single strand confirmation polymorphism (SSCP) detection in exon I of the alpha -lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)

• Published in: Genetics and molecular biology Vol. 29; no. 2; pp. 287 - 289
• Main Authors: Kumar, D, Gupta, N, Ahlawat, SPS, Satyanarayana, R, Sunder, S, Gupta, S C
• Format: Journal Article
• Language: English
• Published: 01.01.2006
• Subjects: Capra hircus
• ISSN: 1415-4757
• DOI: 10.1590/S1415-47572006000200016

The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect alpha -lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the alpha -lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These alpha -lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.