The differential effects of human leukocytic pyrogen/lymphocyte-activating factor, T cell growth factor, and interferon on human natural killer activity

The effects of different lymphocyte- and monocyte-derived mediators on human natural killer (NK) cell activity were examined. Human peripheral blood mononuclear cells (PBMC) or Percoll-enriched large granular lymphocytes (LGL) were preincubated in medium supplemented with lectin-free T cell growth f...

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Published inThe Journal of immunology (1950) Vol. 129; no. 6; pp. 2504 - 2510
Main Authors Dempsey, R A, Dinarello, CA, Mier, J W, Rosenwasser, L J, Allegretta, M, Brown, TE, Parkinson, DR
Format Journal Article
LanguageEnglish
Published 01.01.1982
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Summary:The effects of different lymphocyte- and monocyte-derived mediators on human natural killer (NK) cell activity were examined. Human peripheral blood mononuclear cells (PBMC) or Percoll-enriched large granular lymphocytes (LGL) were preincubated in medium supplemented with lectin-free T cell growth factor (TCGF) preparations lacking interferon (IFN). Cells from these cultures exhibited a dose-dependent augmentation of NK cell activity against K562 leukemia cells, when compared with PBMC not incubated with TCGF. It appears that TCGF is capable of directly stimulating human natural cell-mediated cytotoxicity. The effects of human leukocytic pyrogen/lymphocyte-activating factor (LP/LAF) on NK cell activity depended on the presence of other lymphokines during preincubation. These studies demonstrate that human NK cell activity is subject to regulation by a variety of lymphokines and monokines.
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ISSN:0022-1767