Isolation and characterization of microsatellite loci from the Gila topminnow (Poeciliopsis o. occidentalis) and their utility in guppies (Poecilia reticulata)

The endangered Gila topminnow (Poeciliopsis o. occidentalis) is a small, live-bearing fish inhabitating four isolated watersheds in Arizona. Factors contributing to the decline of what was once the most abundant fish in the Gila River drainage include habitat destruction and/or fragmentation and the...

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Published inMolecular ecology Vol. 7; no. 3; pp. 361 - 363
Main Authors Parker, K M, Hughes, K, Kim, T J, Hedrick, P W
Format Journal Article
LanguageEnglish
Published 01.03.1998
Subjects
Poecilia reticulata
Poeciliopsis occidentalis occidentalis
USA, Arizona
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Summary:The endangered Gila topminnow (Poeciliopsis o. occidentalis) is a small, live-bearing fish inhabitating four isolated watersheds in Arizona. Factors contributing to the decline of what was once the most abundant fish in the Gila River drainage include habitat destruction and/or fragmentation and the introduction of non-native central mosquitofish. Previous genetic studies have shown little variation with one haplotype based on mitochondrial DNA restriction site variation and only two polymorphic allozyme loci out of 25. A recent study of a highly polymorphic MHC class II gene characterized nine different alleles among the four populations. Here we provide information on the microsatellite loci we have developed to examine neutral variation in Gila top-minnows and give data showing their applicability in guppies, Poecilia reticulata.
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ISSN:0962-1083