HEPATO-CURATIVE AND REGENERATIVE POTENTIALS OF WILD OLIVE (Olea ferruginea) FRUIT PULP EXTRACTS AGAINST FLUORIDE-INDUCED TOXICITY IN MICE: A HISTOPATHOLOGICAL STUDY

• Published in: Fluoride Vol. 49; no. 2; p. 112
• Main Authors: Ahmad, Khawaja Raees, Sial, Bushra, Amiruddin, Umara, Bilal, Muhammad Asim, Raees, Kausar, Abbas, Tahir, Kanwal, Muhammad Ali, Ahmad, Syeda Nadia, Andleeb, Shagufta, Ullah, Asmat
• Format: Journal Article
• Language: English
• Published: Dunedin International Society for Fluoride Research, Inc 01.04.2016
• Subjects: Antioxidants; Atherosclerosis; Drinking water; Flavonoids; Fluorides; Food; Fruits; Hepatology; Histopathology; Laboratory animals; Liver; Olives; Oxidative stress; Phenols; Rodents; Stem cells; Vitamin E
• ISSN: 0015-4725; 2253-4083

Ameliorative potentials of wild olive fruit pulp extract (WOFPE) were compared with vitamin E against the hepato-histopathological responses of fluoride exposure (50 ppm in drinking water) in mice. Forty adult males were divided equally into 4 groups: control (C), fluoride (F), fluoride+vitamin E (FE), and fluoride+olive (FO). The mice in the C group were provided F-free drinking water throughout the 15- day study period. The animals in the other 3 groups were given 50 ppm of F- (from NaF) in drinking water for 10 days followed by F-free water for the next 5 days. Additionally, the FE and FO groups were given vitamin E (60 µg in 0.1 mL corn oil) or 0.1 mL WOFPE, respectively, by gavage 12-hourly on days 11-15. Liver samples were obtained from the animals on day 16 after euthanasia. In contrast to the control group, hepato-histopathological signs observed in the F group included peri-central intra-lobular focal hepatic lesions (PIFHL), misaligned hepatic cords with little or no sinusoidal spaces in-between them, and, in the hepatocytes, enlarged nuclei with vague margins and cytoplasmic vacuolations. Although aggregations of oval stem cells were seen in the FE group, PIFHL were also visible. The PIFHL were found to be completely healed in the FO group, and, in addition, large number of oval cells were also seen, both in clumped aggregations and defused between the hepatic cords. Moreover, the juvenile hepatocytes were found to be aligning to produce nascent hepatic cords and epithelial cells were also seen infesting the intra-lobular spaces among the nascent hepatic cords. Analysis of the micrometric data indicated a significant difference (p<0.05) in the mean cross-sectional area (MCSA) of the hepatocytes in the F (498.8 µm2) and FO (350.8 µm2) groups. Moreover they also differed significantly (p<0.05) from the C (419.5 µm2) and FE groups (404.5 µm2). The mean sinusoidal breadth in all four groups (C: 8.62 µm, F: 1.9 µm, FE: 5.02 µm and FO: 3.19 µm) differed significantly (p<0.05) from each other. These findings indicate that subchronic exposure to 50 ppm F in drinking water may cause hepatocytic damage and hepatolobular derangements while WOFPE had better curative and regenerative potentials than a standard natural antioxidant (vitamin E) against these toxicological manifestations.