ISOLATION AND CHARACTERIZATION OF NEW DELHI METALLO-β-LACTAMASE 1-PRODUCING ENTEROBACTER CLOACAE CLINICAL STRAIN IN CHONGQING, CHINA

Carbapenem resistance is an increasing clinical problem worldwide. From a total of 299 Enterobacter cloacae isolates collected from patients at the First Affiliated Hospital of Chongqing Medical University, Chongqing, China between 2007 and 2012, E. cloacae strain 413 from sputum of a 67-year-old fe...

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Published inSoutheast Asian journal of tropical medicine and public health Vol. 48; no. 3; p. 604
Main Authors Zhao, Jinqiu, Chen, Yatang, Sun, Qiu, Shi, Fangjing, Yang, Junjun, Huang, Wenxiang
Format Journal Article
LanguageEnglish
Published Bangkok Central Coordinating Board, SEAMEO-TROPMED Project 01.05.2017
Subjects
Amoxicillin
Antibiotics
Bacteria
Bacterial infections
Carbapenemase
Cefoperazone
Ceftazidime
Chromium
Deoxyribonucleic acid
DNA
DNA sequencing
Drug resistance
E coli
Enterobacter cloacae
Enzymes
Ethylenediaminetetraacetic acids
Fosfomycin
Gram-negative bacteria
Hospitals
Imipenem
Metallo-β-lactamase
Metallography
Pathogens
Penicillin
Piperacillin
Piperacillin-tazobactam
Plasmids
Polymyxin B
Southern blotting
Sputum
Tazobactam
Urine
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Summary:Carbapenem resistance is an increasing clinical problem worldwide. From a total of 299 Enterobacter cloacae isolates collected from patients at the First Affiliated Hospital of Chongqing Medical University, Chongqing, China between 2007 and 2012, E. cloacae strain 413 from sputum of a 67-year-old female demonstrated carbapenemase production based on a modified Hodge test and contained a plasmid (pNDM-1) carrying blaNDM-1 encoding New Delhi metallo-β-lactamase 1. This was confirmed by both Southern blotting and direct DNA sequencing. Production of metallo-β-lactamase was determined by imipenem-EDTA E-test. PCR-based assay showed pNDM-1 also carried aac(6')-Ib-cr, blaCTX-M-14' qnrA, qnrS, and rmtA, consistent with resistance of E. cloacae 413 to amoxicillin, cefoperazone, ceftazidime, penicillin, and piperacillin-tazobactam. E. cloacae 413 was sensitive to fosfomycin and polymyxin B. Pulsed-field gel-electrophoresis of XbaI-digested DNA revealed pNDM-1 belonged to B type. The plasmid could be transferred to Escherichia coli J53, conferring the same antibiogram profile. These findings highlight the spread of NDM-1-carrying bacteria and the necessity for rational use of antibiotics.
ISSN:0125-1562