Detection of Brucella abortus DNA in the reproductive tract of buffalo (Bubalus bubalis) cows

Brucella spp. are the pathogens responsible for brucellosis, which is a cosmopolitan and serious disease for animals and humans. Recent studies have revealed the presence of Brucella DNA in the reproductive tract tissues of buffaloes based on microbiological cultures collected from vaginal swabs. We...

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Published inGenetics and molecular research no. 3; p. 1
Main Authors Ayala, HDM, Monteiro, EMM, Souza, AJS, Filho, ST Rolim, Barbosa, EM, Vale, WG, Filho, E Silva, Pereira, WLA
Format Journal Article
LanguageEnglish
Published Ribeirao Preto Fundacao de Pesquisas Cientificas de Ribeirao Preto 2019
Subjects
Bayesian analysis
Brucella
Brucella abortus
Brucellosis
Complement fixation
Deoxyribonucleic acid
DNA
Lymph nodes
Lymphatic system
Nucleotide sequence
Reproductive system
Uterus
Vagina
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Summary:Brucella spp. are the pathogens responsible for brucellosis, which is a cosmopolitan and serious disease for animals and humans. Recent studies have revealed the presence of Brucella DNA in the reproductive tract tissues of buffaloes based on microbiological cultures collected from vaginal swabs. We tested for Brucella abortus DNA in the uterus, perivaginal lymph nodes, and uterine mucus of buffalo cows naturally infected with Brucella spp., as defined by a positive complement fixation test. Uterine tissue, uterine mucus, and perivaginal lymph node samples from 16 Brucella seropositive female buffaloes were analyzed. Brucella DNA was found in five of the uterine tissue samples, eight of the perivaginal lymph node samples, and 14 of the uterine mucus samples. Furthermore, Bayesian inference analysis indicated that the DNA sequences obtained from these tissues and mucus grouped with corresponding sequences of the B. abortus species.
ISSN:1676-5680
DOI:10.4238/gmr18376