A Rabbit model for evaluating the efficacy of recombinant adenovirus vaccine expressing E0-E2 antigens of classical swine fever virus
This study investigated the efficacy of a recombinant adenovirus vaccine expressing E0-E2 antigens (rAd-E0E2) of the classical swine fever virus (CSFV) and determined its minimum effective dose in rabbits. Forty rabbits were randomly divided into five groups. Three groups were immunized intramuscula...
Saved in:
Published in | Wētchasān sattawaphāet Vol. 47; no. 3; p. 363 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Bangkok
Chulalongkorn University, Faculty of Veterinary Science
01.09.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | This study investigated the efficacy of a recombinant adenovirus vaccine expressing E0-E2 antigens (rAd-E0E2) of the classical swine fever virus (CSFV) and determined its minimum effective dose in rabbits. Forty rabbits were randomly divided into five groups. Three groups were immunized intramuscularly with rAd-E0-E2 at doses of 107-°, 106-° and 105-° IFU, respectively. CSFV C strain was administered to one group and saline to another group (negative control). Fourteen days after immunization, the animals were challenged intramuscularly with CSFV C strain. Stereotypical thermal responses were monitored to assess the protective efficacy of the vaccine. No typical thermal response was observed in either the 107.0 IFU or CSFV C strain groups. Mean spleen weight to body weight ratios in these groups were 0.055% and 0.05%, respectively, no evidence of hyperemia was observed in the splenic tissue, and no CSFV C strain was detected in the spleens by either RT-PCR or indirect fluorescent antibody (IFA) test. In the 106.0 IFU group, 6/8 rabbits showed typical thermal response and the spleen weight to body weight ratio was 0.074%. Further, in 6/8 rabbits, the splenic tissues showed hyperemia and virus was detected by IFA. In both 105.° IFU and control groups, all animals developed fever and the spleen weight to body weight ratios were 0.099% and 0.102%, respectively. Severe hyperemia was observed and the virus was detected in the splenic tissue by RT-PCR and IFA. In conclusion, rAd-E0-E2 confers protection against CSFV C strain infection, and a rabbit model for evaluating the efficacy of the vaccine has been successfully established. The minimum effective dose in rabbits in this study was 1070 IFU. |
---|---|
ISSN: | 0125-6491 |