Angiotensin-I converting enzyme (ACE) inhibitory peptides from chicken skin gelatin hydrolysate and its antihypertensive effect in spontaneously hypertensive rats

Chicken skin gelatin hydrolysates and peptides with angiotensin converting enzyme inhibitory (ACEI) activity were produced enzymatically using alcalase, pronase E, and collagenase before fractionation into <2, <5, and <10 kDa fractions. The fraction with the highest ACEI activity was furthe...

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Bibliographic Details
Published inInternational food research journal Vol. 26; no. 3; pp. 903 - 911
Main Authors Ahmad, W A N Wan, Abd Gani, S S, Rokhani, F Z
Format Journal Article
LanguageEnglish
Published Selangor Universiti Putra Malaysia, Faculty of Food Science & Technology 01.06.2019
Subjects
Angiotensin
Antihypertensives
Biochemistry
Blood pressure
Captopril
Chickens
Chromatography
Collagen
Collagenase
Conversion
Enzymes
Filtration
Food
Food industry
Fractionation
Gel filtration
Gelatin
High performance liquid chromatography
Hydrolysates
Hypertension
Liquid chromatography
Molecular biology
Oral administration
Peptides
Peptidyl-dipeptidase A
Pharmaceutical industry
Poultry
Pronase
Proteins
Skin
Subtilisin
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Summary:Chicken skin gelatin hydrolysates and peptides with angiotensin converting enzyme inhibitory (ACEI) activity were produced enzymatically using alcalase, pronase E, and collagenase before fractionation into <2, <5, and <10 kDa fractions. The fraction with the highest ACEI activity was further purified sequentially using gel filtration chromatography RP-HPLC. The ACEI activity of <2, <5 and <10 kDa hydrolysate fractions were 69.64, 76.80 and 87.69%, respectively. Gel filtration chromatography of <2 kDa fraction produced peptides with ACEI activity (92%), and showed a similarity to the synthetic drug captopril (93%). The relative molecular mass range of the <2 kDa peptide fraction was 300-900 Da, comprising mainly Glu, H.Pro, Gly, Ala, Pro and Lys. Further separation using HPLC revealed a fraction with the sequence of one peptide: Gly-Pro-Ile-Gly-Pro-Pro-Ser-Gly-Gly-Phe-Asp (IC50, 0.04 mg/mL). The antihypertensive effect of the purified peptide was supported by spontaneously hypertensive rats (SHR). Oral administration of a 15 mg/kg dose of the purified peptide decreased systolic blood pressure significantly for 24 h. These results suggest that the purified peptide derived from chicken skin gelatin hydrolysate has potential antihypertensive properties which can be used as antihypertensive agent in the food and pharmaceutical industries.
ISSN:1985-4668
2231-7546