Pluripotent Until Needed

BIRTH CERTIFICATE When researchers are preparing to reprogram an adult cell to pluripotency, or when they've just discovered a new iPSC, it is important to verify that the line has no genetic aberrations, such as a trisomy or a loss-of-function mutation, before and after reprogramming. [...]exp...

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Bibliographic Details
Published inThe Scientist Vol. 27; no. 4; p. 60
Main Author Mole, Beth Marie
Format Trade Publication Article
LanguageEnglish
Published Midland LabX Media Group 01.04.2013
Subjects
Arrays
Chromosomes
Cytogenetics
Genomes
Methods
Mutation
Proteins
Stem cells
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Summary:BIRTH CERTIFICATE When researchers are preparing to reprogram an adult cell to pluripotency, or when they've just discovered a new iPSC, it is important to verify that the line has no genetic aberrations, such as a trisomy or a loss-of-function mutation, before and after reprogramming. [...]experts recommend checking for genetic abnormalities in stem cell lines every 15 to 20 passages, depending on the protocol used. [...]custom-made SNP microarrays allow researchers to design DNA probes for a genome region of interest to their study.
ISSN:0890-3670
1547-0806