HIF - 1 α may promote glycolysis in psoriasis vulgaris via upregulation of CD147 and GLUT1

• Published in: Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban Vol. 46; no. 4; pp. 333 - 344
• Main Authors: Tang, Wen, Long, Tingting, Li, Fangfang, Peng, Cong, Zhao, Shuang, Chen, Xiang, Su, Juan
• Format: Journal Article
• Language: English
• Published: China Central South University Press 28.04.2021
• Subjects: Basigin; Glucose Transporter Type 1; Glycolysis; Humans; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Psoriasis - genetics; Transcriptional Activation; Up-Regulation; hypoxia-inducible factor-1α; CD147; glucose transporter 1; psoriasis; glycolysis
• ISSN: 1672-7347
• DOI: 10.11817/j.issn.1672-7347.2021.200010

To analyze the expressions and distributions of hypoxia-inducible factor-1α (HIF-1α), CD147, and glucose transporter 1 (GLUT1) in epidermis from psoriasis vulgaris and normal people, and to explore the associations among these proteins and their roles in hypoxic HaCaT cell line. The expression levels of HIF-1α, CD147, and GLUT1 were determined by immunohistochemistry staining in skin biopsies from 48 psoriasis vularis patients and 33 healthy subjects. Cobalt chloride (CoCl ) was added into the culture media of HaCaT cells to mimic hypoxia while RNA interference and transfection technologies were used to explore the association among these proteins by quantitative real-time polymerase chain reaction and Western blotting. Glycolytic capacity was detected by ATP and lactate measurements. HIF-1α, CD147, and GLUT1 were highly expressed and the glycolytic capacity was increased in lesions of psoriasis vulgaris; HIF-1α upregulated the expression of CD147 and GLUT1, increased the lactate production and decreased the ATP level in CoCl -treated HaCaT cells, while CD147 and GLUT1 directly or indirectly bound to each other. Glycolytic capacity increases in the injured keratinocytes of psoriasis vulgaris, suggesting that HIF-1α, CD147, and GLUT1 are associated with glycolysis, which can be considered as the promising targets for psoriasis therapy.