Effect of temperature, cholesterol and nerve tissue on multiplication of armadillo M. leprae

The effect of temperature, nerve tissue and certain constituents of the medium on multiplication of armadillo M. leprae was studied using Hanks BSS. An equal or better growth was seen at 30 degrees C and 10 degrees C compared to 37 degrees C. Multiplication was also seen at -20 degrees C. Adding cho...

Full description

Saved in:
Bibliographic Details
Published inIndian journal of leprosy Vol. 61; no. 4; p. 453
Main Author Bhatia, V N
Format Journal Article
LanguageEnglish
Published India 01.10.1989
Subjects
Animals
Armadillos - microbiology
Blood
Cattle
Cholesterol - pharmacology
Culture Media
Cystine - pharmacology
Mycobacterium leprae - growth & development
Nerve Tissue
Temperature
Thioglycolates - pharmacology
Online AccessGet more information

Cover

More Information
Summary:The effect of temperature, nerve tissue and certain constituents of the medium on multiplication of armadillo M. leprae was studied using Hanks BSS. An equal or better growth was seen at 30 degrees C and 10 degrees C compared to 37 degrees C. Multiplication was also seen at -20 degrees C. Adding cholesterol, foetal calf serum, cystine-HCl, sodium thioglycollate or nerve suspension and covering medium with liquid paraffin each showed beneficial effect. Hanks containing foetal calf serum, cholesterol with sodium thioglycollate or cystine-hydrochloride showed maximum multiplication. These combinations may be used for testing additional factors for further improvement of the medium.
ISSN:0254-9395