Phytosterols from Spondias mombin Linn with Antimycobacterial Activities

The growing problems of tuberculosis have led to the search for new anti-Mtb agents from higher plants. The stem bark of was evaluated for its activity against (H37Rv strain). Bioassay-guided fractionation of the methanol extract was carried out by Vacuum Liquid Chromatography (VLC) on Silica gel (2...

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Bibliographic Details
Published inAfrican journal of biomedical research Vol. 16; no. 1; pp. 19 - 24
Main Authors Olugbuyiro, J A O, Moody, J O, Hamann, M T
Format Journal Article
LanguageEnglish
Published Nigeria 01.01.2013
Subjects
Spondias mombin
antimycobacterial
mombintane I
Mycobacterium tuberculosis
mombintane II
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Summary:The growing problems of tuberculosis have led to the search for new anti-Mtb agents from higher plants. The stem bark of was evaluated for its activity against (H37Rv strain). Bioassay-guided fractionation of the methanol extract was carried out by Vacuum Liquid Chromatography (VLC) on Silica gel (230-400 mesh) and purification was done using HPLC and TLC. antimycobacterial susceptibility was performed by a fluorometric microplate alamar blue assay (MABA) and percentage mycobacterial inhibition was calculated. The structures of the isolated compounds were established by spectroscopic analysis. The active VLC fraction exhibited 91% inhibition against H37Rv at a concentration of 40 μg/mL. The HPLC fraction SMi-15 containing compounds and showed 92.8% inhibition against . Two new antimycobacterial phytosterols were isolated from the stem bark of and the structures were identified as mombintane I ( ) and mombintane II ( ). The stem bark extractives of contain antitubercular principles of the class phytosterol and support an important potential of triterpenoids.
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ISSN:1119-5096