A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method

The concentration of plasma sialic acid was estimated using the modified chemical method and the more sensitive enzymatic method in 20 subjects with impaired glucose tolerance and 20 control subjects. The mean sialic acid concentration values of the control subjects and subjects with impaired glucos...

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Published inSoutheast Asian journal of tropical medicine and public health Vol. 36; no. 4; pp. 1011 - 1013
Main Authors MUNIANDY, S, QVIST, R, ZAINI, A, CHINNA, K, ISMAIL, I. S
Format Journal Article
LanguageEnglish
Published Bangkok Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network 01.07.2005
Central Coordinating Board, SEAMEO-TROPMED Project
Subjects
Biological and medical sciences
Chemistry Techniques, Analytical - economics
Chemistry Techniques, Analytical - methods
Enzyme-Linked Immunosorbent Assay - methods
General aspects
Humans
Malaysia
Medical sciences
Periodic Acid
Reagent Kits, Diagnostic
Reproducibility of Results
Resorcinols
Sialic Acids - analysis
Asia
Malaysia
Assay
Human
Tropical medicine
Sialic acid
Comparative study
Blood plasma
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Summary:The concentration of plasma sialic acid was estimated using the modified chemical method and the more sensitive enzymatic method in 20 subjects with impaired glucose tolerance and 20 control subjects. The mean sialic acid concentration values of the control subjects and subjects with impaired glucose tolerance using the enzymatic method were 1.747 +/- 0.047 and 2.583 +/- 0.070 mmole/l and 1.753 +/- 0.067 and 2.591 +/- 1.02 mmole/l for the chemical method. The intra-assay coefficient of variation for the control subjects and for the subjects with impaired glucose tolerance were 1.963% and 1.583%, respectively, for the enzymatic assay and 2.728% and 2.431%, respectively, for the chemical assay. The inter-assay coefficient of variation for the control subjects and for the subjects with impaired glucose tolerance were 2.686% and 2.723% for the enzymatic assay, and 3.819% and 3.95% for the chemical assay. Since the values do not differ significantly, the chemical assay is a cost effective method that can be used in large epidemiological studies.
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ISSN:0125-1562