WITHAFERIN A INDUCES APOPTOSIS IN RAT C6 GLIOMA CELLS THROUGH REGULATING NF-KB NUCLEAR TRANSLOCATION AND ACTIVATION OF CASPASE CASCADE

• Published in: African journal of traditional, complementary, and alternative medicines Vol. 14; no. 2; pp. 319 - 324
• Main Authors: Hou, Wei-Chen, Miao, Xiao-Hui, Ma, Lian-Jun, Bai, Xiao-Xue, Liu, Qun, Song, Lei
• Format: Journal Article
• Language: English
• Published: Nigeria African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2017
• Subjects: Animals; Antineoplastic Agents, Phytogenic - pharmacology; Antineoplastic Agents, Phytogenic - therapeutic use; Apoptosis; bcl-2-Associated X Protein - metabolism; Biological Transport; Caspases - metabolism; Cell Line, Tumor; Cell Proliferation; DNA Fragmentation - drug effects; Enzyme Activation; Glioma - drug therapy; Glioma - metabolism; Inflammation - etiology; Inflammation - prevention & control; NF-kappa B - metabolism; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Proto-Oncogene Proteins c-bcl-2 - metabolism; Rats; Withania - chemistry; Withanolides - pharmacology; Withanolides - therapeutic use; anti-inflammatory; Withaferin A; apoptosis; C6 glioma cells; anti-proliferative; caspase
• ISSN: 0189-6016; 2505-0044
• DOI: 10.21010/ajtcam.v14i2.33

The demand for the chemopreventive drug from the plant source is increasing in recent times, owing to its various biological activities without any adverse effect. The intention of this current study was to examine the anti-glioma effect of Withaferin A (WFA) on C6 glioma cell line model. C6 glioma cells were administrated with different concentration of WFA (50, 100, 200 and 500 μg/mL) and DMSO (control) group to examine its anti-proliferative, anti-inflammatory and pro-apoptotic activities. Treatment with WFA showed a significant decline in the glioma cell count in a dose-dependent manner and thus proving its anti-proliferative effect. Similarly, inflammatory markers were also substantially lowered upon treatment with different concentration of WFA. However, DNA fragmentation and apoptotic markers like Caspase-3 and 9 were concomitantly enhanced after co-cultured with different concentration of WFA and thus exhibiting its cytotoxicity efficacy. Furthermore, the protein expression of Bcl2 and Bax were markedly downregulated and upregulated respectively; upon treatment with WFA on C6 glioma cells. The outcome of this study evidently demonstrates that C6 glioma cells co-cultured with increased concentration of WFA, showed an anti-proliferative, anti-inflammatory and pro-apoptotic effect in a dose-dependent fashion.