Interleukin-10 and Transforming Growth Factor Beta1 Gene Polymorphisms in Chronic Heart Failure

• Published in: Acta bio-medica : Atenei Parmensis Vol. 90; no. 2; pp. 221 - 227
• Main Authors: Mahmoudi, Mohammad Jafar, Hedayat, Mona, Taghvaei, Mohammad, Harsini, Sara, Nematipour, Ebrahim, Rezaei, Nima, Farhadi, Elham, Mahmoudi, Maryam, Sadr, Maryam, Esfahanian, Nilufar, Nourijelyani, Keramat, Amirzargar, Ali Akbar
• Format: Journal Article
• Language: English
• Published: Italy Mattioli 1885 23.05.2019
• Subjects: Aged; Case-Control Studies; Chi-Square Distribution; Chronic Disease; Female; Gene Expression Regulation; Genetic Markers - physiology; Genotype; Heart Failure - diagnosis; Heart Failure - genetics; Humans; Interleukin-10 - genetics; Iran; Male; Middle Aged; Odds Ratio; Original; Polymorphism, Single Nucleotide; Prognosis; Reference Values; Severity of Illness Index; Transforming Growth Factor beta2 - genetics; Iran
• ISSN: 0392-4203; 2531-6745
• DOI: 10.23750/abm.v90i2.6681

As cytokines, including interleukin-10 (IL-10) and transforming growth factor beta 1(TGF-β1) seem to contribute towards the pathogenesis of chronic heart failure (CHF), this study was performed to assess the associations of certain single nucleotide polymorphisms (SNPs) of these genes in a case control study. This investigation was carried out to determine the frequency of alleles, genotypes and haplotypes of TGF-β1 and IL-10 single-nucleotide polymorphisms (SNPs) in 57 Iranian patients with CHF compared with 140 healthy subjects using polymerase chain reaction with sequence-specific primers method. Results of the analyzed data divulged a negative association for both TGF-β1 GC genotype at codon 25 (P=0.047) and CT genotype at codon 10 (P=0.018) and CHF proneness. Although, TGF-β1 CC genotype at codon 10 was found to be positively associated with CHF (P=0.011). Moreover, the frequency of IL-10 (-1082, -819, -592) ATA haplotype and TGF-β1 (codon 10, codon 25) TG haplotype were significantly lower in the patients group (P=0.004 and P=0.040, respectively), while TGF-β1 (codon 10, codon 25) CG haplotype was overrepresented in patients with CHF (P=0.007). Cytokine gene polymorphisms might affect vulnerability to CHF. Particular genotypes and haplotypes in IL-10 and TGF-β1 genes could render individuals more susceptible to CHF.