Salivary immunoglobulins in recipients of bone marrow grafts. III: A longitudinal follow-up of CMV specific antibodies

• Published in: Bone marrow transplantation (Basingstoke) Vol. 17; no. 2; pp. 237 - 241
• Main Authors: CHAUSHU, G, CHAUSHU, S, SLAVIN, S, OR, R, GARFUNKEL, A. A, YEFENOF, E
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 01.02.1996
• Subjects: Adolescent; Adult; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Antibodies, Viral - analysis; Antibodies, Viral - blood; Antibody Specificity; B-Lymphocytes - transplantation; Biological and medical sciences; Bone Marrow Transplantation - adverse effects; Bone Marrow Transplantation - immunology; Bone marrow, stem cells transplantation. Graft versus host reaction; Child; Cytomegalovirus - immunology; Cytomegalovirus - physiology; Cytomegalovirus Infections - etiology; Cytomegalovirus Infections - immunology; Cytomegalovirus Infections - prevention & control; Cytomegalovirus Infections - transmission; Enzyme-Linked Immunosorbent Assay; Female; Follow-Up Studies; Graft vs Host Disease - immunology; human cytomegalovirus; Humans; Immunocompromised Host; Immunoglobulin G - analysis; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunoglobulin M - analysis; Immunoglobulin M - blood; Immunoglobulin M - immunology; Immunoglobulins, Intravenous - pharmacokinetics; Immunoglobulins, Intravenous - therapeutic use; Male; Medical sciences; Middle Aged; Parotid Gland - secretion; Plasma Cells - transplantation; Saliva - immunology; Salivary Proteins and Peptides - analysis; Time Factors; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Virus Activation; Human; Cytomegalovirus; Immunoglobulins; Transfusion; Antibody; Herpesviridae; Homograft; Betaherpesvirinae; Infection; Virus; Follow up study; Viral disease; Bone marrow; Complication; Graft; Saliva
• ISSN: 0268-3369; 1476-5365

Cytomegalovirus (CMV) infection is a major complication of BMT. The oral cavity is a common route for CMV infection, whose protection is provided by salivary anti-CMV antibodies. We developed an ELISA assay for the detection of CMV-specific antibodies in parotid saliva. Saliva of patients receiving BMT from CMV-positive donors was transiently reconstituted with IgG and IgA anti-CMV antibodies shortly after transplantation. The concentration of these antibodies gradually decreased during the 2 months after transplantation and increased again around day 80. A remarkable rise in the salivary concentrations of IgG and IgM anti-CMV was observed shortly after i.v. administration of Sandoglobulin. These results demonstrate, for the first time accurate monitoring of CMV-specific antibodies in saliva using a quantitative ELISA assay. The study suggests that secretion of CMV-specific antibodies in saliva of immunocompromised patients can be reconstituted by donor-derived B and plasma cells transferred with the BM or by i.v. administration of pooled Ig.