Identification of clinical isolates of Leptospira spp by pulsed field gel-electrophoresis and microscopic agglutination test

Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and relia...

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Published inSoutheast Asian journal of tropical medicine and public health Vol. 38; no. 1; pp. 97 - 103
Main Authors NAIGOWIT, Pimjai, CHAROENCHAI, Sathapana, BIAKLANG, Mayurachat, SEENA, Umaporn, WANGROONGSARB, Piyada, SAWANPANYALERT, Pathom, WARACHIT, Paijit
Format Journal Article
LanguageEnglish
Published Bangkok Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network 2007
Central Coordinating Board, SEAMEO-TROPMED Project
Subjects
Agglutination Tests
Bacterial Typing Techniques
Biological and medical sciences
DNA, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
General aspects
Humans
Leptospira - classification
Leptospira - genetics
Leptospira - isolation & purification
Leptospira interrogans - genetics
Leptospira interrogans - isolation & purification
Leptospirosis - diagnosis
Leptospirosis - genetics
Leptospirosis - microbiology
Medical sciences
Reproducibility of Results
Sensitivity and Specificity
Serotyping
Thailand
Thailand
Pulsed field electrophoresis
Spirochaetales
Leptospiraceae
Bacteria
Tropical medicine
Agglutination test
Identification
Clinical isolate
Leptospira
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Summary:Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and reliable than MAT.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0125-1562