Co-localization of LHβ and FSHβ mRNAS in the porcine anterior pituitary by in situ hybridization with biotinylated probes

• Published in: Biochemical and biophysical research communications Vol. 154; no. 1; pp. 80 - 84
• Main Authors: LIU, Y. C, KATO, Y, INOUE, K, TANAKA, S, KUROSUMI, K
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 15.07.1988
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Biotin; Follicle Stimulating Hormone - genetics; Follicle Stimulating Hormone, beta Subunit; Fundamental and applied biological sciences. Psychology; Indicators and Reagents; Luteinizing Hormone - genetics; Nucleic Acid Hybridization; Pituitary Gland, Anterior - cytology; Pituitary Gland, Anterior - metabolism; Protein hormones. Growth factors. Cytokines; Proteins; RNA, Messenger - genetics; Swine; Hormone; Glycoproteins; Gonadotropin; Hybridization; Subunit; Proteins; Tissue; Vertebrata; Mammalia; Messenger RNA; Complementary DNA; Microscopy; Pituitary gland; FSH; Molecular probe; LH; Localization; Nucleic acid
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(88)90652-3

The localizations of mRNAs encoding LH beta and FSH beta in porcine pituitary were investigated by in situ hybridization technique. Biotinylated porcine LH beta and FSH beta cDNA probes were used on frozen sections of paraformaldehyde-fixed pituitary specimens. Hybridizations to both mRNAs were observed specifically in cytoplasm with unstained nuclei. Furthermore, cells hybridized for LH beta mRNA were demonstrated to be identical to those for FSH beta mRNA. This study provided the first morphological evidence that both gonadotropin beta genes are expressed in the same cell.