Conformational ensembles in Klebsiella pneumoniae FimH impact uropathogenesis

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 121; no. 39; p. e2409655121
• Main Authors: Lopatto, Edward D B, Pinkner, Jerome S, Sanick, Denise A, Potter, Robert F, Liu, Lily X, Bazán Villicaña, Jesús, Tamadonfar, Kevin O, Ye, Yijun, Zimmerman, Maxwell I, Gualberto, Nathaniel C, Dodson, Karen W, Janetka, James W, Hunstad, David A, Hultgren, Scott J
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 24.09.2024
• Subjects: Adhesins, Bacterial - chemistry; Adhesins, Bacterial - genetics; Adhesins, Bacterial - metabolism; Adhesins, Escherichia coli - chemistry; Adhesins, Escherichia coli - genetics; Adhesins, Escherichia coli - metabolism; Adhesives; Affinity; Binding; Binding Sites; Biological Sciences; Crystallography; Crystallography, X-Ray; E coli; Female; Fimbriae Proteins - chemistry; Fimbriae Proteins - genetics; Fimbriae Proteins - metabolism; Fimbriae, Bacterial - metabolism; Gene sequencing; Humans; Klebsiella; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - metabolism; Mannose; Mannose - metabolism; Models, Molecular; Morbidity; Phylogeny; Pilin; Protein Binding; Protein Conformation; Protein Domains; Quality of life; Urinary tract; Urinary tract infections; Urinary Tract Infections - microbiology; Urogenital system; mechanism of bacterial colonization; UTI; bacterial pathogenesis
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2409655121

is an important pathogen causing difficult-to-treat urinary tract infections (UTIs). Over 1.5 million women per year suffer from recurrent UTI, reducing quality of life and causing substantial morbidity and mortality, especially in the hospital setting. Uropathogenic (UPEC) is the most prevalent cause of UTI. Like UPEC, relies on type 1 pili, tipped with the mannose-binding adhesin FimH, to cause cystitis. However, FimH is a poor binder of mannose, despite a mannose-binding pocket identical to UPEC FimH. FimH is composed of two domains that are in an equilibrium between tense (low-affinity) and relaxed (high-affinity) conformations. Substantial interdomain interactions in the tense conformation yield a low-affinity, deformed mannose-binding pocket, while domain-domain interactions are broken in the relaxed state, resulting in a high-affinity binding pocket. Using crystallography, we identified the structural basis by which domain-domain interactions direct the conformational equilibrium of FimH, which is strongly shifted toward the low-affinity tense state. Removal of the pilin domain restores mannose binding to the lectin domain, thus showing that poor mannose binding by FimH is not an inherent feature of the mannose-binding pocket. Phylogenetic analyses of genomes found that FimH sequences are highly conserved. However, we surveyed a collection of isolates from patients with long-term indwelling catheters and identified isolates that possessed relaxed higher-binding FimH variants, which increased fitness in bladder infection models, suggesting that long-term residence within the urinary tract may select for higher-binding FimH variants.