Restriction Endonuclease Sst12I from a Strain of Streptomyces Recognizing the Nucleotide Sequence 5"-CTGCAG-3"

A new restriction endonuclease Sst12I belonging to type II and recognizing the sequence 5"-CTGCAG-3" was isolated from the bacterial strain Streptomycessp. St-12. The enzyme hydrolyzes DNA between adenine and guanine residues; thus, it is a true isoschizomer of restrictase PstI. In contras...

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Published inApplied biochemistry and microbiology Vol. 38; no. 1; p. 20
Main Authors Ushakova, T A, Puchkova, L I, Gutorov, V V, Kuvshinov, V N, Repin, V E
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.01.2002
Subjects
Bacteria
Deoxyribonucleic acid
DNA
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Summary:A new restriction endonuclease Sst12I belonging to type II and recognizing the sequence 5"-CTGCAG-3" was isolated from the bacterial strain Streptomycessp. St-12. The enzyme hydrolyzes DNA between adenine and guanine residues; thus, it is a true isoschizomer of restrictase PstI. In contrast to PstI, the restriction endonuclease Sst12I hydrolyses DNA both at 37 and 55°C and remains active after long-term storage.[PUBLICATION ABSTRACT]
ISSN:0003-6838
1608-3024
DOI:10.1023/A:1013288202420