Using amino acid analysis to determine absorptivity constants: A validation case study using bovine serum albumin

Amino acid analysis of well-recovered residues offers an easy way to calculate the absorptivity constant for a known protein. The method provides an absolute measure of protein concentration, free from interference from water, excipients, and bound salts. This article demonstrates a qualified method...

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Bibliographic Details
Published inBiopharm International Vol. 16; no. 2; p. 30
Main Authors Anders, John C, Parten, Benne F, Petrie, Glenn E, Marlowe, Robert L, McEntire, John E
Format Trade Publication Article
LanguageEnglish
Published Monmouth Junction MultiMedia Healthcare Inc 01.02.2003
Subjects
Amino acids
Aqueous solutions
Case studies
Cattle
Methods
Peptides
Proteins
Salt
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Summary:Amino acid analysis of well-recovered residues offers an easy way to calculate the absorptivity constant for a known protein. The method provides an absolute measure of protein concentration, free from interference from water, excipients, and bound salts. This article demonstrates a qualified method for determining protein content by AAA.
ISSN:1542-166X
1939-1862