PCR-ELISA. II: Analysis of Bifidobacterium populations in human faecal samples from a consumption trial with Bifidobacterium lactis Bb-12 and a galacto-oligosaccharide preparation

• Published in: Systematic and applied microbiology Vol. 25; no. 2; pp. 249 - 258
• Main Authors: MALINEN, Erja, MÄTIÖ, Jaana, SALMITIE, Merja, ALANDER, Minna, SAARELA, Maria, PALVA, Airi
• Format: Journal Article
• Language: English
• Published: Jena Elsevier 01.08.2002; Elsevier Science Ltd
• Subjects: Animals; Bacterial Typing Techniques; Bacteriological methods and techniques used in bacteriology; Bacteriology; Bifidobacterium - classification; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Biological and medical sciences; DNA, Bacterial - analysis; DNA, Bacterial - classification; DNA, Bacterial - genetics; Enzyme-Linked Immunosorbent Assay - methods; Feces - microbiology; Feeding; Fundamental and applied biological sciences. Psychology; Galactose - administration & dosage; Humans; Ingestion; Microbiology; Milk - microbiology; Nucleic Acid Hybridization; Oligonucleotide Probes; Oligosaccharides - administration & dosage; Oligosaccharides - analysis; Oligosaccharides - classification; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Polymerase Chain Reaction - methods; Probiotics - administration & dosage; Sensitivity and Specificity; Human; Probiotic; Actinomycetaceae; Ribosomal DNA; Bifidobacterium longum; Method; Actinomycetales; Polymerase chain reaction; Sensitivity; Specificity; Gastrointestinal diseases; Bacteria; Actinomycetes; Oligonucleotide; Detection; ELISA assay
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1078/0723-2020-00117

A PCR-ELISA method was extended for detection of most common Bifidobacterium species in humans and applied to a feeding trial including administration of Bifidobacterium lactis Bb-12 and galacto-oligosaccharide (GOS)-containing syrup as probiotic and prebiotic preparations, respectively. For PCR-ELISA, oligonucleotide probes based on 16S rDNA sequences were designed and tested for specificity and sensitivity with nine different bifidobacterial species followed by analysis of faecal samples. Bifidobacteria were monitored for their fluctuations during and after the feeding trial. Bifidobacterium longum was the most common species found in the faecal samples, followed by B. adolescentis and B. bifidum. During ingestion of the probiotic B. lactis Bb-12, the strain appeared in the faeces but was absent again one week after finishing of the trial. The species that were observed in the faecal samples taken prior to the feeding experiments persisted also in samples derived from the pre-feeding and feeding periods. The most consistent change observed was the decrease in the relative amount of B. longum in the test group ingesting either B. lactis Bb-12 alone or in combination with GOS-syrup. Since the amounts of B. longum increased again in the post-feeding sample with these subjects, it may suggest that to some extent B. lactis Bb-12 is able to transiently replace B. longum.