Prevention of ischemia-reperfusion injury in rat ovarian tissue with the on-off method

Ischemia is defined as cell death caused by insufficient perfusion of the tissue due to reduction in arterial or venous blood flow, depletion of cellular energy storages, and accumulation of toxic metabolites. The positive effects of controlled reperfusion are known and are used clinically. But the...

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Published inJournal of physiology and pharmacology : an official journal of the Polish Physiological Society Vol. 62; no. 5; p. 575
Main Authors Ingec, M, Isaoglu, U, Yilmaz, M, Calik, M, Polat, B, Alp, H H, Kurt, A, Gundogdu, C, Suleyman, H
Format Journal Article
LanguageEnglish
Published Poland 01.10.2011
Subjects
Animals
Antioxidants - metabolism
DNA Damage
Female
Glutathione - metabolism
Ischemia - complications
Ischemia - metabolism
Ischemia - pathology
Ischemic Preconditioning - methods
Malondialdehyde - metabolism
Ovary - blood supply
Ovary - metabolism
Ovary - pathology
Rats
Rats, Wistar
Reperfusion - methods
Reperfusion Injury - etiology
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Superoxide Dismutase - metabolism
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Summary:Ischemia is defined as cell death caused by insufficient perfusion of the tissue due to reduction in arterial or venous blood flow, depletion of cellular energy storages, and accumulation of toxic metabolites. The positive effects of controlled reperfusion are known and are used clinically. But the positive effects of controlled reperfusion on ovarian tissue have not been seen in the literature yet. The biochemical and histopathological comparative investigation of rat ovaries that were experimentally exposed to ischemia (IG), ischemia-reperfusion (I/R), and ischemia-controlled reperfusion (ICR) was aimed. Forty rats were divided into four groups (10 rats per group). First group: 3 h ischemia by vascular clips on ovarian tissue. Second group: 3 h ischemia + 1 h reperfusion. Third group: 3 h ischemia + 1 h controlled reperfusion (on-off method: controlled reperfusion by opening and closing the clips (on/off) in 10-second intervals, for 5 times for a total of 100 seconds). Fourth group: healthy rats. Biochemical (tGSH, MDA, and DNA damage level and SOD activity) and histopathological analysis were performed. The highest glutathione and superoxide dismutase measurements were found in ischemia/controlled reperfusion group among the ischemia or ischemia/reperfusion groups. Similarly the damage indicators (malondialdehyde, DNA damage level and histopathological damage grade) were the lowest in ischemia/controlled reperfusion group. These results indicate that controlled reperfusion can be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia for various reasons (ovarian torsion, tumor, etc.).
ISSN:1899-1505