Capture of dengue virus type 3 using anionic polymer-coated magnetic beads

• Published in: International journal of molecular medicine Vol. 28; no. 4; pp. 625 - 628
• Main Authors: Sakudo, Akikazu, Masrinoul, Promsin, Tanaka, Yasuharu, Ikuta, Kazuyoshi
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.10.2011
• Subjects: Aedes - virology; Animals; Blotting, Western; Cell Line; Dengue Virus - isolation & purification; Magnetics; Polymers - chemistry; Reverse Transcriptase Polymerase Chain Reaction
• ISSN: 1107-3756; 1791-244X
• DOI: 10.3892/ijmm.2011.733

Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes is the most effective way of controlling DENV infections, detection of DENVs in mosquitoes is limited by the low sensitivity of available detection methods. We here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). The beads were incubated with cell culture medium of DENV-3-infected mosquito cells, then separated from the supernatant by applying a magnetic field and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase chain reaction, which detected the presence of DENV-3 genomic RNA on the beads, and Western blotting, which determined the major DENV-3 envelope protein on the beads. Therefore, this capture method may enable an improvement in DENV-3 detection.