Studies on nuclear staining by the fluorescent antibody method. I. Non-immunological factors leading to nuclear fluorescence

The causes of non-specific nuclear staining in the fluorescent antibody method were investigated. Minute amounts of labelled basic proteins, present in labelled sera, stain nuclei in both direct and indirect methods. The attraction of these basic proteins is caused by electrostatical forces, binding...

Full description

Saved in:
Bibliographic Details
Published inImmunology Vol. 19; no. 1; pp. 1 - 10
Main Authors Bruchhausen, D, Hofermann, R, von Mayersbach, H
Format Journal Article
LanguageEnglish
Published England 01.07.1970
Subjects
Animals
Cell Nucleus
Electricity
Fluorescence
Fluorescent Antibody Technique
Fluorescent Dyes
Liver - cytology
Mice
Muramidase
Protamines
Protein Binding
Staining and Labeling
Online AccessGet full text

Cover

More Information
Summary:The causes of non-specific nuclear staining in the fluorescent antibody method were investigated. Minute amounts of labelled basic proteins, present in labelled sera, stain nuclei in both direct and indirect methods. The attraction of these basic proteins is caused by electrostatical forces, binding basic items to the nuclei like basic dyes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0019-2805
1365-2567