The bovine immune response to Brucella abortus. II. Elimination of some sporadic serological reactions by chelation of divalent cations

The standard agglutination tests for detecting antibody to Brucella abortus were modified by addition of chelating agents (EDTA and EGTA) to the antigens. Approximately 80% of "singleton" agglutination test reactions, negative on the diagnostic complement fixation test, obtained with cattl...

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Published inCanadian journal of comparative medicine Vol. 43; no. 4; pp. 420 - 425
Main Authors Nielsen, K, Samagh, B S, Speckmann, G, Stemshorn, B
Format Journal Article
LanguageEnglish
Published Canada 01.10.1979
Subjects
Agglutination Tests
Animals
Antibodies, Bacterial - analysis
Brucella abortus - immunology
Brucellosis, Bovine - diagnosis
Cations, Divalent
Cattle - immunology
Edetic Acid - pharmacology
Egtazic Acid - pharmacology
Ethylene Glycols - pharmacology
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Abstract The standard agglutination tests for detecting antibody to Brucella abortus were modified by addition of chelating agents (EDTA and EGTA) to the antigens. Approximately 80% of "singleton" agglutination test reactions, negative on the diagnostic complement fixation test, obtained with cattle sera were eliminated while no decrease in titer was apparent when sera from B. abortus infected or vaccinated cattle were tested.
AbstractList The standard agglutination tests for detecting antibody to Brucella abortus were modified by addition of chelating agents (EDTA and EGTA) to the antigens. Approximately 80% of "singleton" agglutination test reactions, negative on the diagnostic complement fixation test, obtained with cattle sera were eliminated while no decrease in titer was apparent when sera from B. abortus infected or vaccinated cattle were tested.
Author Stemshorn, B
Samagh, B S
Speckmann, G
Nielsen, K
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References 101470 - Infect Immun. 1978 Sep;21(3):994-1002
405088 - Can J Comp Med. 1977 Apr;41(2):152-9
5575145 - Acta Vet Scand. 1971;12(1):86-94
825095 - Berl Munch Tierarztl Wochenschr. 1976 Aug 1;89(15):300-2
810011 - Acta Vet Scand. 1975;16(2):318-20
14125895 - Am J Vet Res. 1964 Mar;25:325-8
14125896 - Am J Vet Res. 1964 Mar;25:329-32
13682980 - Am J Vet Res. 1961 May;22:564-9
13050878 - Am J Vet Res. 1953 Apr;14(51):192-4
6067975 - Vet Rec. 1967 May 27;80(21):612-20
13444570 - Am J Vet Res. 1957 Jul;18(68):550-5
17649570 - Can J Comp Med Vet Sci. 1966 Oct;30(10):270-8
4112241 - Can J Microbiol. 1972 Apr;18(4):449-56
14864628 - Proc Soc Exp Biol Med. 1951 Jul;77(3):469-72
4234793 - Can J Comp Med. 1968 Oct;32(4):598-9
13050879 - Am J Vet Res. 1953 Apr;14(51):195-7
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Snippet The standard agglutination tests for detecting antibody to Brucella abortus were modified by addition of chelating agents (EDTA and EGTA) to the antigens....
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SubjectTerms Agglutination Tests
Animals
Antibodies, Bacterial - analysis
Brucella abortus - immunology
Brucellosis, Bovine - diagnosis
Cations, Divalent
Cattle - immunology
Edetic Acid - pharmacology
Egtazic Acid - pharmacology
Ethylene Glycols - pharmacology
Title The bovine immune response to Brucella abortus. II. Elimination of some sporadic serological reactions by chelation of divalent cations
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