The bovine immune response to Brucella abortus. II. Elimination of some sporadic serological reactions by chelation of divalent cations

The standard agglutination tests for detecting antibody to Brucella abortus were modified by addition of chelating agents (EDTA and EGTA) to the antigens. Approximately 80% of "singleton" agglutination test reactions, negative on the diagnostic complement fixation test, obtained with cattl...

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Bibliographic Details
Published inCanadian journal of comparative medicine Vol. 43; no. 4; pp. 420 - 425
Main Authors Nielsen, K, Samagh, B S, Speckmann, G, Stemshorn, B
Format Journal Article
LanguageEnglish
Published Canada 01.10.1979
Subjects
Agglutination Tests
Animals
Antibodies, Bacterial - analysis
Brucella abortus - immunology
Brucellosis, Bovine - diagnosis
Cations, Divalent
Cattle - immunology
Edetic Acid - pharmacology
Egtazic Acid - pharmacology
Ethylene Glycols - pharmacology
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Summary:The standard agglutination tests for detecting antibody to Brucella abortus were modified by addition of chelating agents (EDTA and EGTA) to the antigens. Approximately 80% of "singleton" agglutination test reactions, negative on the diagnostic complement fixation test, obtained with cattle sera were eliminated while no decrease in titer was apparent when sera from B. abortus infected or vaccinated cattle were tested.
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ISSN:0008-4050