Polydispersity of human articular cartilage proteoglycan antigens

Studies were undertaken to define more fully the antigenic properties of human articular cartilage proteoglycans, in anticipation of its potential contribution to alterations arising in diseased states and following cartilage transplantation. Proteoglycans, extracted from normal, adult articular car...

Full description

Saved in:
Bibliographic Details
Published inJournal of rheumatology Vol. 3; no. 4; p. 390
Main Authors Herman, J H, Dennis, M V
Format Journal Article
LanguageEnglish
Published Canada 01.12.1976
Subjects
Adult
Antigens - analysis
Cartilage, Articular - immunology
Electrophoresis, Polyacrylamide Gel
Epitopes
Erythrocytes - immunology
Hemagglutination Tests
Humans
Immunodiffusion
Middle Aged
Proteoglycans - immunology
Online AccessGet more information

Cover

More Information
Summary:Studies were undertaken to define more fully the antigenic properties of human articular cartilage proteoglycans, in anticipation of its potential contribution to alterations arising in diseased states and following cartilage transplantation. Proteoglycans, extracted from normal, adult articular cartilage by dissociative measures, were subjected to purification by cesium density gradient ultracentrifugation, under conditions facilitating both molecular aggregation and dissociation. A polydisperse population of reactive determinants was observed in immunodiffusion and hemagglutination inhibition systems, employing proteoglycan specific antisera on gradient fractions. Highly aggregated proteoglycan species appeared to contain potentially masked antigenic determinants, which were revealed after guanidine dissociation but not hyaluronidase digestion. Polyacrilamide disc gel electrophoresis in sodium dodecyl sulfate, in conjunction with disc elution experiments, confirmed proteoglycan antigenic polydispersity.
ISSN:0315-162X