Simultaneous determination of four beta-lactam antibiotics in milk by solid-phase extraction-micellar electrokinetic chromatography

A solid-phase extraction (SPE)-micellar electrokinetic chromatography (MEKC) method was developed for the simultaneous determination of four beta-lactam antibiotics (ampicillin, amoxicillin, penicillin V and cephalexin) in spiked milk samples. The milk samples were extracted by SPE after protein pre...

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Bibliographic Details
Published inSepu Vol. 26; no. 6; p. 682
Main Authors Zhang, Qi, Ye, Nengsheng, Gu, Xuexin, Hao, Xiaoli, Liu, Ni
Format Journal Article
LanguageChinese
Published China 01.11.2008
Subjects
Animals
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - isolation & purification
beta-Lactams - analysis
beta-Lactams - isolation & purification
Buffers
Calibration
Chromatography, Micellar Electrokinetic Capillary - methods
Food Analysis - methods
Food Contamination - analysis
Hydrogen-Ion Concentration
Limit of Detection
Linear Models
Milk - chemistry
Phosphates - chemistry
Reproducibility of Results
Solid Phase Extraction - methods
Time Factors
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Summary:A solid-phase extraction (SPE)-micellar electrokinetic chromatography (MEKC) method was developed for the simultaneous determination of four beta-lactam antibiotics (ampicillin, amoxicillin, penicillin V and cephalexin) in spiked milk samples. The milk samples were extracted by SPE after protein precipitation. The analysis was performed by MEKC at 18 kV with phosphate buffer containing sodium dodecyl sulfate (SDS) and the detection was carried out at 210 nm. The four antibiotics could be baseline-separated less than 9 min. The linearities in 0.5 - 20 mg/L could be constructed with the correlation coefficients between 0.994 3 - 0.997 6, and the limits of detection were 0.16 - 0.20 mg/L. The average recoveries of antibiotics from milk were over 70% except amoxicillin. The method is sensitive, specific and easy for the determination of beta-lactam antibiotic residues in milk.
ISSN:1000-8713