Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteoblast-like cells: comparison with TGF-beta 1

The biologic effects of recombinant human bone morphogenetic protein-2b (BMP-2b = BMP-4) were studied and compared with transforming growth factor-beta 1 (TGF-beta 1) in fetal rat osteoblast-like (ROB) cells. Similar to the effects of TGF-beta 1, BMP-2b stimulated DNA and collagen synthesis as well...

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Bibliographic Details
Published inJournal of bone and mineral research Vol. 6; no. 12; p. 1387
Main Authors Chen, T L, Bates, R L, Dudley, A, Hammonds, Jr, R G, Amento, E P
Format Journal Article
LanguageEnglish
Published United States 01.12.1991
Subjects
Alkaline Phosphatase - metabolism
Animals
Bone Morphogenetic Proteins
Cell Division - drug effects
Cells, Cultured
Collagen - biosynthesis
DNA - biosynthesis
Growth Substances - pharmacology
Humans
Osteoblasts - cytology
Osteoblasts - drug effects
Proteins - metabolism
Proteins - pharmacology
Rats
Rats, Inbred Strains
Thymidine - metabolism
Transforming Growth Factor beta - pharmacology
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Summary:The biologic effects of recombinant human bone morphogenetic protein-2b (BMP-2b = BMP-4) were studied and compared with transforming growth factor-beta 1 (TGF-beta 1) in fetal rat osteoblast-like (ROB) cells. Similar to the effects of TGF-beta 1, BMP-2b stimulated DNA and collagen synthesis as well as protein accumulation. Unlike TGF-beta 1, which inhibited alkaline phosphatase activity, BMP-2b enhanced enzyme activity eight-to ninefold over the control level. The present study demonstrates direct actions of BMP-2b on bone-associated cells to stimulate osteogenic phenotypes in vitro and provides a cellular mechanism for the induction of bone formation by BMP-2b in vivo.
ISSN:0884-0431