Binding of acridine orange and ethidium bromide to DNA in phage lambda

Binding of acridine orange and ethidium bromide to phage lambda C1857 has been studied by fluorescence and absorption measurements. It is shown that these dyes are strongly bound to DNA in the phage and are not bound to phage proteins. 50% of interphage DNA is stainable.

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Bibliographic Details
Published inBiofizika Vol. 27; no. 2; p. 222
Main Authors Shurdov, M A, Kishchenko, G P
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1982
Subjects
Acridine Orange
Bacteriophage lambda - metabolism
DNA, Viral - metabolism
Ethidium
Kinetics
Spectrometry, Fluorescence
Spectrophotometry
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Summary:Binding of acridine orange and ethidium bromide to phage lambda C1857 has been studied by fluorescence and absorption measurements. It is shown that these dyes are strongly bound to DNA in the phage and are not bound to phage proteins. 50% of interphage DNA is stainable.
ISSN:0006-3029