Human CCAAT displacement protein is homologous to the Drosophila homeoprotein, cut

Human CCAAT displacement protein (CDP), a putative repressor of developmentally regulated gene expression, was purified from HeLa cells by DNA binding-site affinity chromatography. cDNA encoding CDP was obtained by immunoscreening a lambda gt11 library with antibody raised against purified protein....

Full description

Saved in:
Bibliographic Details
Published inNature genetics Vol. 1; no. 1; pp. 50 - 55
Main Authors Neufeld, E J, Skalnik, D G, Lievens, P M, Orkin, S H
Format Journal Article
LanguageEnglish
Published United States 01.04.1992
Subjects
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA - genetics
Drosophila
Drosophila - genetics
Drosophila Proteins
Gene Expression
HeLa Cells
Homeodomain Proteins
Humans
Molecular Sequence Data
Nerve Tissue Proteins - genetics
Nuclear Proteins - genetics
Repressor Proteins - genetics
Sequence Homology, Amino Acid
Species Specificity
Transcription Factors
Online AccessGet full text

Cover

More Information
Summary:Human CCAAT displacement protein (CDP), a putative repressor of developmentally regulated gene expression, was purified from HeLa cells by DNA binding-site affinity chromatography. cDNA encoding CDP was obtained by immunoscreening a lambda gt11 library with antibody raised against purified protein. The deduced primary amino acid sequence of CDP reveals remarkable homology to Drosophila cut with respect to the presence of a unique homeodomain and "cut repeats". As cut participates in determination of cell fate in several tissues in Drosophila, the similarity predicts a broad role for CDP in mammalian development.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:1061-4036
DOI:10.1038/ng0492-50