Microtubule Reorganization During ABA-Induced Stomatal Closure in Arabidopsis

Plants must cope with diverse environmental stresses during growth and development, among which drought is one of the most concerning global threats. Recent studies have shown that the disassembly of the microtubule cytoskeleton plays an essential role during ABA-induced stomatal closure in response...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2604; p. 249
Main Authors Dou, Liru, Wang, Xiangfeng, Mao, Tonglin
Format Journal Article
LanguageEnglish
Published United States 2023
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Summary:Plants must cope with diverse environmental stresses during growth and development, among which drought is one of the most concerning global threats. Recent studies have shown that the disassembly of the microtubule cytoskeleton plays an essential role during ABA-induced stomatal closure in response to drought stress. To facilitate studies on the mechanisms of ABA-induced microtubule rearrangement during stomatal closure, we describe procedures for observing guard cells treated with ABA, visualizing the microtubule cytoskeleton in guard cells, and their subsequent quantitative analysis. We include both representative images and the quantification results to illustrate these experiments.
ISSN:1940-6029
DOI:10.1007/978-1-0716-2867-6_19