Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment

The discovery of opioid receptor expression on immune cells has originated a large research activity on the possible modulation by opioid drugs of immune system responses. In this chapter we describe an easy methodology useful to obtain information about the potential immunomodulatory activity of op...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2201; p. 199
Main Authors Franchi, Silvia, Castelli, Mara, Moretti, Sarah, Panerai, Alberto, Sacerdote, Paola
Format Journal Article
LanguageEnglish
Published United States 2021
Subjects
Analgesics, Opioid - immunology
Analgesics, Opioid - pharmacology
Animals
Cytokines - analysis
Cytokines - drug effects
Immune System Phenomena
Immunologic Factors - analysis
Immunologic Factors - pharmacology
Immunomodulation - drug effects
Immunomodulation - immunology
Macrophages - drug effects
Macrophages - metabolism
Mice
Morphine - adverse effects
Morphine - metabolism
Morphine - pharmacology
Primary Cell Culture - methods
Receptors, Opioid - immunology
Lipopolysaccharide
Opioid-induced immunosuppression
Morphine
Murine macrophages
Cytokines
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Summary:The discovery of opioid receptor expression on immune cells has originated a large research activity on the possible modulation by opioid drugs of immune system responses. In this chapter we describe an easy methodology useful to obtain information about the potential immunomodulatory activity of opioid drugs. An in vivo treatment schedule is used, and macrophages are studied for their ability to release different cytokines.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0884-5_18