In Vitro Generation of Human Antibody-Secreting Cells Through the Stimulation of PBMCs with Dengue Virus Particles

The Dengue pathophysiology has had several aspects determined over the years. However, some points remain elusive, such as the metabolic factors that regulate the massive B cell differentiation into antibody-secreting cells observed in Dengue patients. In this chapter, we describe an in vitro method...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2409; p. 235
Main Authors Bonezi, Vivian, Cataneo, Allan Henrique Depieri, Wowk, Pryscilla Fanini, Silveira, Eduardo L V
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
Antibody-Producing Cells
Dengue
Dengue Virus
Humans
Leukocytes, Mononuclear
Virion
Cell culture
Antibody-secreting cell differentiation
Dengue virus
PBMCs
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Summary:The Dengue pathophysiology has had several aspects determined over the years. However, some points remain elusive, such as the metabolic factors that regulate the massive B cell differentiation into antibody-secreting cells observed in Dengue patients. In this chapter, we describe an in vitro method capable of mimicking this Dengue-induced cell expansion. More specifically, this approach allows dengue virus-stimulated peripheral blood mononuclear cells (PBMCs) from healthy individuals to enhance the frequency of phenotypical and functional antibody-secreting cells (ASCs) after 7 days of culture. A manuscript recently published by Bonezi and colleagues displays results generated through this methodology.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1879-0_16