Hydrophilic Interaction Liquid Chromatography (HILIC) Enrichment of Glycopeptides Using PolyHYDROXYETHYL A

Glycosylation of proteins is an important post-translational modification that plays a role in a wide range of biological processes, including immune response, intercellular signaling, inflammation, and host-pathogen interaction. Abnormal protein glycosylation has been correlated with various diseas...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2762; p. 267
Main Authors Goli, Mona, Jiang, Peilin, Fowowe, Mojibola, Hakim, Md Abdul, Mechref, Yehia
Format Journal Article
LanguageEnglish
Published United States 2024
Subjects
Chromatography, Liquid - methods
Glycopeptides - analysis
Glycosylation
Hydrophobic and Hydrophilic Interactions
Protein Processing, Post-Translational
TopTip C18 desalting
LC-MS/MS
Glycopeptides
HILIC enrichment
Online AccessGet more information

Cover

More Information
Summary:Glycosylation of proteins is an important post-translational modification that plays a role in a wide range of biological processes, including immune response, intercellular signaling, inflammation, and host-pathogen interaction. Abnormal protein glycosylation has been correlated with various diseases. However, the study of protein glycosylation remains challenging due to its low abundance, microheterogeneity of glycosylation sites, and low ionization efficiency. During the past decade, several methods for enrichment and for isolation of glycopeptides from biological samples have been developed and successfully employed in glycoproteomics research. In this chapter, we discuss the sample preparation protocol and the strategies for effectively isolating and enriching glycopeptides from biological samples, using PolyHYDROXYETHYL A as a hydrophilic interaction liquid chromatography (HILIC) enrichment technique.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3666-4_16