Digital Holographic Microscopy to Assess Cell Behavior

Digital holographic microscopy is an imaging technique particularly well suited to the study of living cells in culture, as no labeling is required and computed phase maps produce high contrast, quantitative pixel information. A full experiment involves instrument calibration, cell culture quality c...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2644; p. 247
Main Authors Bazow, Brad, Lam, Van K, Phan, Thuc, Chung, Byung Min, Nehmetallah, George, Raub, Christopher B
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Cell Culture Techniques
Cell Line
Holography
Humans
Image Interpretation, Computer-Assisted - methods
Microscopy - methods
Cell motility
Image processing
Microscopy
Cell lines
Machine learning
Holography
Quantitative phase
Cell morphology
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Summary:Digital holographic microscopy is an imaging technique particularly well suited to the study of living cells in culture, as no labeling is required and computed phase maps produce high contrast, quantitative pixel information. A full experiment involves instrument calibration, cell culture quality checks, selection and setup of imaging chambers, a sampling plan, image acquisition, phase and amplitude map reconstruction, and parameter map post-processing to extract information about cell morphology and/or motility. Each step is described below, focusing on results from imaging four human cell lines. Several post-processing approaches are detailed, with an aim of tracking individual cells and dynamics of cell populations.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3052-5_16