FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids
Recent technological developments have led to widespread applications of large-scale transcriptomics-based sequencing methods to identify genotype-to-cell type associations. Here we describe a fluorescence-activated cell sorting (FACS)-based sequencing method to utilize CRISPR/Cas9 edited mosaic cer...
Saved in:
Published in | Methods in molecular biology (Clifton, N.J.) Vol. 2683; p. 193 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
2023
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Summary: | Recent technological developments have led to widespread applications of large-scale transcriptomics-based sequencing methods to identify genotype-to-cell type associations. Here we describe a fluorescence-activated cell sorting (FACS)-based sequencing method to utilize CRISPR/Cas9 edited mosaic cerebral organoids to identify or validate genotype-to-cell type associations. Our approach is high-throughput and quantitative and uses internal controls to enable comparisons of the results across different antibody markers and experiments. |
---|---|
ISSN: | 1940-6029 |
DOI: | 10.1007/978-1-0716-3287-1_15 |