FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids

Recent technological developments have led to widespread applications of large-scale transcriptomics-based sequencing methods to identify genotype-to-cell type associations. Here we describe a fluorescence-activated cell sorting (FACS)-based sequencing method to utilize CRISPR/Cas9 edited mosaic cer...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2683; p. 193
Main Authors Murray, Liam, Olson, Meagan N, Barton, Nathaniel, Dawes, Pepper, Chan, Yingleong, Lim, Elaine T
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
FACS
Genotype to cell type
Sequencing
Cerebral organoids
oFlowSeq
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Summary:Recent technological developments have led to widespread applications of large-scale transcriptomics-based sequencing methods to identify genotype-to-cell type associations. Here we describe a fluorescence-activated cell sorting (FACS)-based sequencing method to utilize CRISPR/Cas9 edited mosaic cerebral organoids to identify or validate genotype-to-cell type associations. Our approach is high-throughput and quantitative and uses internal controls to enable comparisons of the results across different antibody markers and experiments.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3287-1_15