Functional Assays in 3D ObaCell® Fat-on-a-Chip Cultures: Lipolysis and Glucose Uptake Assays

Advancements in three-dimensional in vitro cultures pose a need for modification of established two-dimensional culture functional assay methods. Application of three-dimensional in vitro models in drug screening and target validation, specifically in the development of compounds targeting adipose m...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2783; p. 323
Main Authors Hamel, Katie, Rogers, Emma, Gimble, Jeffrey M, Frazier, Trivia, Sanchez, Cecilia
Format Journal Article
LanguageEnglish
Published United States 2024
Subjects
Three-dimensional cell culture
Glucose uptake
Fat-on-a-chip
Human-derived hydrogel
Lipolysis
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Summary:Advancements in three-dimensional in vitro cultures pose a need for modification of established two-dimensional culture functional assay methods. Application of three-dimensional in vitro models in drug screening and target validation, specifically in the development of compounds targeting adipose metabolic activity, requires optimization of current glucose uptake and lipolysis assay protocols to effectively measure adipocyte function in a three-dimensional platform. This chapter describes the establishment of three-dimensional cultures using Obatala Sciences' human-derived hydrogel, maintenance and treatment of the cultures, and evaluation of compound response via lipolysis and glucose uptake assays.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3762-3_23